학술논문
IFN-stimulated Gene Expression, Type 2 Inflammation, and Endoplasmic Reticulum Stress in Asthma
Document Type
article
Author
Bhakta, Nirav R; Christenson, Stephanie A; Nerella, Srilaxmi; Solberg, Owen D; Nguyen, Christine P; Choy, David F; Jung, Kyle L; Garudadri, Suresh; Bonser, Luke R; Pollack, Joshua L; Zlock, Lorna T; Erle, David J; Langelier, Charles; Derisi, Joseph L; Arron, Joseph R; Fahy, John V; Woodruff, Prescott G
Source
American Journal of Respiratory and Critical Care Medicine. 197(3)
Subject
Language
Abstract
RationaleQuantification of type 2 inflammation provided a molecular basis for heterogeneity in asthma. Non-type 2 pathways that contribute to asthma pathogenesis are not well understood.ObjectivesTo identify dysregulated pathways beyond type 2 inflammation.MethodsWe applied RNA sequencing to airway epithelial brushings obtained from subjects with stable mild asthma not on corticosteroids (n = 19) and healthy control subjects (n = 16). Sequencing reads were mapped to human and viral genomes. In the same cohort, and in a separate group with severe asthma (n = 301), we profiled blood gene expression with microarrays.Measurements and main resultsIn airway brushings from mild asthma on inhaled corticosteroids, RNA sequencing yielded 1,379 differentially expressed genes (false discovery rate