학술논문
Analysis of somatic mutations in 131 human brains reveals aging-associated hypermutability
Document Type
article
Author
Bae, Taejeong; Fasching, Liana; Wang, Yifan; Shin, Joo Heon; Suvakov, Milovan; Jang, Yeongjun; Norton, Scott; Dias, Caroline; Mariani, Jessica; Jourdon, Alexandre; Wu, Feinan; Panda, Arijit; Pattni, Reenal; Chahine, Yasmine; Yeh, Rebecca; Roberts, Rosalinda C; Huttner, Anita; Kleinman, Joel E; Hyde, Thomas M; Straub, Richard E; Walsh, Christopher A; Urban, Alexander E; Leckman, James F; Weinberger, Daniel R; Vaccarino, Flora M; Abyzov, Alexej; Park, Peter J; Sestan, Nenad; Weinberger, Daniel; Moran, John V; Gage, Fred H; Gleeson, Joseph; Mathern, Gary; Courchesne, Eric; Roy, Subhojit; Chess, Andrew J; Akbarian, Schahram; Bizzotto, Sara; Coulter, Michael; D’Gama, Alissa; Ganz, Javier; Hill, Robert; Huang, August Yue; Khoshkhoo, Sattar; Kim, Sonia; Lee, Alice; Lodato, Michael; Maury, Eduardo A; Miller, Michael; Borges-Monroy, Rebeca; Rodin, Rachel; Zhou, Zinan; Bohrson, Craig; Chu, Chong; Cortes-Ciriano, Isidro; Dou, Yanmei; Galor, Alon; Gulhan, Doga; Kwon, Minseok; Luquette, Joe; Sherman, Maxwell; Viswanadham, Vinay; Jones, Attila; Rosenbluh, Chaggai; Cho, Sean; Langmead, Ben; Thorpe, Jeremy; Erwin, Jennifer; Jaffe, Andrew; McConnell, Michael; Narurkar, Rujuta; Paquola, Apua; Shin, Jooheon; Straub, Richard; Molitor, Cindy; Peters, Mette; Linker, Sara; Reed, Patrick; Wang, Meiyan; Urban, Alexander; Zhou, Bo; Zhu, Xiaowei; Serres Amero, Aitor; Juan, David; Lobon, Irene; Marques-Bonet, Tomas; Solis Moruno, Manuel; Garcia Perez, Raquel; Povolotskaya, Inna; Soriano, Eduardo; Antaki, Danny; Averbuj, Dan
Source
Science. 377(6605)
Subject
Language
Abstract
We analyzed 131 human brains (44 neurotypical, 19 with Tourette syndrome, 9 with schizophrenia, and 59 with autism) for somatic mutations after whole genome sequencing to a depth of more than 200×. Typically, brains had 20 to 60 detectable single-nucleotide mutations, but ~6% of brains harbored hundreds of somatic mutations. Hypermutability was associated with age and damaging mutations in genes implicated in cancers and, in some brains, reflected in vivo clonal expansions. Somatic duplications, likely arising during development, were found in ~5% of normal and diseased brains, reflecting background mutagenesis. Brains with autism were associated with mutations creating putative transcription factor binding motifs in enhancer-like regions in the developing brain. The top-ranked affected motifs corresponded to MEIS (myeloid ectopic viral integration site) transcription factors, suggesting a potential link between their involvement in gene regulation and autism.