학술논문

Microorganisms Move a Short Distance into an Almond Orchard from an Adjacent Upwind Poultry Operation
Document Type
article
Source
Applied and Environmental Microbiology. 86(15)
Subject
Microbiology
Biological Sciences
Air Microbiology
Animal Husbandry
Animals
California
Dust
Enterobacteriaceae
Escherichia coli
Microbiota
Plant Leaves
Poultry
Prunus dulcis
RNA
Bacterial
RNA
Ribosomal
16S
Salmonella
Soil Microbiology
Trees
Wind
almond
bioaerosol
dust
microbiome
orchard
phyllosphere
poultry
Medical microbiology
Language
Abstract
Over a 2-year period, drag swabs of orchard soil surface and air, soil, and almond leaf samples were collected in an almond orchard adjacent to (35 m from the first row of trees) and downwind from a poultry operation and in two almond orchards (controls) that were surrounded by other orchards. Samples were evaluated for aerobic plate count, generic Escherichia coli, other coliforms, the presence of Salmonella, bacterial community structure (analyzed through sequencing of the 16S rRNA gene), and amounts of dry solids (dust) on leaf surfaces on trees 0, 60, and 120 m into each orchard. E. coli was isolated from 41 of 206 (20%) and 1 of 207 (0.48%) air samples in the almond-poultry and control orchards, respectively. Salmonella was not isolated from any of the 529 samples evaluated. On average, the amount of dry solids on leaves collected from trees closest to the poultry operation was more than 2-fold greater than from trees 120 m into the orchard or from any of the trees in the control orchards. Members of the family Staphylococcaceae-often associated with poultry-were, on average, significantly (P