학술논문
Systemic cytokines and GlycA discriminate disease status and predict corticosteroid response in HTLV-1-associated neuroinflammation.
Document Type
article
Author
Assone, Tatiane; Menezes, Soraya Maria; de Toledo Gonçalves, Fernanda; Folgosi, Victor Angelo; da Silva Prates, Gabriela; Dierckx, Tim; Braz, Marcos; Smid, Jerusa; Haziot, Michel E; Marcusso, Rosa MN; Dahy, Flávia E; Vanderlinden, Evelien; Claes, Sandra; Schols, Dominique; Bruhn, Roberta; Murphy, Edward L; Penalva de Oliveira, Augusto César; Daelemans, Dirk; Vercauteren, Jurgen; Casseb, Jorge; Van Weyenbergh, Johan
Source
Journal of neuroinflammation. 19(1)
Subject
Language
Abstract
BackgroundHTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP) is an incapacitating neuroinflammatory disorder for which no disease-modifying therapy is available, but corticosteroids provide some clinical benefit. Although HAM/TSP pathogenesis is not fully elucidated, older age, female sex and higher proviral load are established risk factors. We investigated systemic cytokines and a novel chronic inflammatory marker, GlycA, as possible biomarkers of immunopathogenesis and therapeutic response in HAM/TSP, and examined their interaction with established risk factors.Patients and methodsWe recruited 110 People living with HTLV-1 (PLHTLV-1, 67 asymptomatic individuals and 43 HAM/TSP patients) with a total of 946 person-years of clinical follow-up. Plasma cytokine levels (IL-2, IL-4, IL-6, IL-10, IL-17A, IFN-γ, TNF) and GlycA were quantified by Cytometric Bead Array and 1NMR, respectively. Cytokine signaling and prednisolone response were validated in an independent cohort by nCounter digital transcriptomics. We used multivariable regression, machine learning algorithms and Bayesian network learning for biomarker identification.ResultsWe found that systemic IL-6 was positively correlated with both age (r = 0.50, p