학술논문
scMicrobe PTA: Near Complete Genomes from Single Bacterial Cells
Document Type
article
Author
Source
bioRxiv. 5(02-13)
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Language
Abstract
Microbial genomes produced by single-cell amplification are largely incomplete. Here, we show that primary template amplification (PTA), a novel single-cell amplification technique, generated nearly complete genomes from three bacterial isolate species. Furthermore, taxonomically diverse genomes recovered from aquatic and soil microbiomes using PTA had a median completeness of 81%, whereas genomes from standard amplification approaches were usually