학술논문
Megahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers
Document Type
article
Author
Holmes, Susannah; Kirkwood, Henry J; Bean, Richard; Giewekemeyer, Klaus; Martin, Andrew V; Hadian-Jazi, Marjan; Wiedorn, Max O; Oberthür, Dominik; Marman, Hugh; Adriano, Luigi; Al-Qudami, Nasser; Bajt, Saša; Barák, Imrich; Bari, Sadia; Bielecki, Johan; Brockhauser, Sandor; Coleman, Mathew A; Cruz-Mazo, Francisco; Danilevski, Cyril; Dörner, Katerina; Gañán-Calvo, Alfonso M; Graceffa, Rita; Fanghor, Hans; Heymann, Michael; Frank, Matthias; Kaukher, Alexander; Kim, Yoonhee; Kobe, Bostjan; Knoška, Juraj; Laurus, Torsten; Letrun, Romain; Maia, Luis; Messerschmidt, Marc; Metz, Markus; Michelat, Thomas; Mills, Grant; Molodtsov, Serguei; Monteiro, Diana CF; Morgan, Andrew J; Münnich, Astrid; Peña Murillo, Gisel E; Previtali, Gianpietro; Round, Adam; Sato, Tokushi; Schubert, Robin; Schulz, Joachim; Shelby, Megan; Seuring, Carolin; Sellberg, Jonas A; Sikorski, Marcin; Silenzi, Alessandro; Stern, Stephan; Sztuk-Dambietz, Jola; Szuba, Janusz; Trebbin, Martin; Vagovic, Patrick; Ve, Thomas; Weinhausen, Britta; Wrona, Krzysztof; Xavier, Paul Lourdu; Xu, Chen; Yefanov, Oleksandr; Nugent, Keith A; Chapman, Henry N; Mancuso, Adrian P; Barty, Anton; Abbey, Brian; Darmanin, Connie
Source
Nature Communications. 13(1)
Subject
Language
Abstract
The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz (MHz) repetition rates. Previous work has shown that it is possible to use consecutive X-ray pulses to collect diffraction patterns from individual crystals. Here, we exploit the MHz pulse structure of the European XFEL to obtain two complete datasets from the same lysozyme crystal, first hit and the second hit, before it exits the beam. The two datasets, separated by