학술논문
Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways
Document Type
article
Author
Klein, Hannah L; Bačinskaja, Giedrė; Che, Jun; Cheblal, Anais; Elango, Rajula; Epshtein, Anastasiya; Fitzgerald, Devon M; Gómez-González, Belén; Khan, Sharik R; Kumar, Sandeep; Leland, Bryan A; Marie, Léa; Mei, Qian; Miné-Hattab, Judith; Piotrowska, Alicja; Polleys, Erica J; Putnam, Christopher D; Radchenko, Elina A; Saada, Anissia Ait; Sakofsky, Cynthia J; Shim, Eun Yong; Stracy, Mathew; Xia, Jun; Yan, Zhenxin; Yin, Yi; Aguilera, Andrés; Argueso, Juan Lucas; Freudenreich, Catherine H; Gasser, Susan M; Gordenin, Dmitry A; Haber, James E; Ira, Grzegorz; Jinks-Robertson, Sue; King, Megan C; Kolodner, Richard D; Kuzminov, Andrei; Lambert, Sarah AE; Lee, Sang Eun; Miller, Kyle M; Mirkin, Sergei M; Petes, Thomas D; Rosenberg, Susan M; Rothstein, Rodney; Symington, Lorraine S; Zawadzki, Pawel; Kim, Nayun; Lisby, Michael; Malkova, Anna
Source
Microbial Cell. 6(1)
Subject
Language
Abstract
Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.