학술논문
Replication of Integrative Data Analysis for Adipose Tissue Dysfunction, Low-Grade Inflammation, Postprandial Responses and OMICs Signatures in Symptom-Free Adults
Document Type
article
Author
Gallegos-Cabriales, Esther C; Rodriguez-Ayala, Ernesto; Laviada-Molina, Hugo A; Nava-Gonzalez, Edna J; Salinas-Osornio, Rocío A; Orozco, Lorena; Leal-Berumen, Irene; Castillo-Pineda, Juan Carlos; Gonzalez-Lopez, Laura; Escudero-Lourdes, Claudia; Cornejo-Barrera, Judith; Escalante-Araiza, Fabiola; Huerta-Avila, Eira E; Buenfil-Rello, Fatima A; Peschard, Vanessa-Giselle; Silva, Eliud; Veloz-Garza, Rosa A; Martinez-Hernandez, Angelica; Barajas-Olmos, Francisco M; Molina-Segui, Fernanda; Gonzalez-Ramirez, Lucia; Arjona-Villicaña, Ruy D; Hernandez-Escalante, Victor M; Gaytan-Saucedo, Janeth F; Vaquera, Zoila; Acebo-Martinez, Monica; Murillo-Ramirez, Areli; Diaz-Tena, Sara P; Figueroa-Nuñez, Benigno; Valencia-Rendon, Melesio E; Garzon-Zamora, Rafael; Viveros-Paredes, Juan Manuel; Valdovinos-Chavez, Salvador B; Comuzzie, Anthony G; Haack, Karin; Thorsell, Ashley A; Han, Xianlin; Cole, Shelley A; Bastarrachea, Raul A
Source
Biology. 10(12)
Subject
Language
Abstract
We previously reported preliminary characterization of adipose tissue (AT) dysfunction through the adiponectin/leptin ratio (ALR) and fasting/postprandial (F/P) gene expression in subcutaneous (SQ) adipose tissue (AT) biopsies obtained from participants in the GEMM study, a precision medicine research project. Here we present integrative data replication of previous findings from an increased number of GEMM symptom-free (SF) adults (N = 124) to improve characterization of early biomarkers for cardiovascular (CV)/immunometabolic risk in SF adults with AT dysfunction. We achieved this goal by taking advantage of the rich set of GEMM F/P 5 h time course data and three tissue samples collected at the same time and frequency on each adult participant (F/P blood, biopsies of SQAT and skeletal muscle (SKM)). We classified them with the presence/absence of AT dysfunction: low (1) ALR. We also examined the presence of metabolically healthy (MH)/unhealthy (MUH) individuals through low-grade chronic subclinical inflammation (high sensitivity C-reactive protein (hsCRP)), whole body insulin sensitivity (Matsuda Index) and Metabolic Syndrome criteria in people with/without AT dysfunction. Molecular data directly measured from three tissues in a subset of participants allowed fine-scale multi-OMIC profiling of individual postprandial responses (RNA-seq in SKM and SQAT, miRNA from plasma exosomes and shotgun lipidomics in blood). Dynamic postprandial immunometabolic molecular endophenotypes were obtained to move towards a personalized, patient-defined medicine. This study offers an example of integrative translational research, which applies bench-to-bedside research to clinical medicine. Our F/P study design has the potential to characterize CV/immunometabolic early risk detection in support of precision medicine and discovery in SF individuals.