학술논문
Minimally Invasive Saliva Testing to Monitor Norovirus Infection in Community Settings
Document Type
article
Author
Pisanic, Nora; Ballard, Sarah-Blythe; Colquechagua, Fabiola D; François, Ruthly; Exum, Natalie; Yori, Pablo Peñataro; Schwab, Kellogg J; Granger, Douglas A; Detrick, Barbara; Olortegui, Maribel Paredes; Mayta, Holger; Sánchez, Gerardo J; Gilman, Robert H; Heaney, Christopher D; Vinjé, Jan; Kosek, Margaret N
Source
The Journal of Infectious Diseases. 219(8)
Subject
Language
Abstract
BackgroundNorovirus is a leading cause of acute gastroenteritis worldwide. Routine norovirus diagnosis requires stool collection. The goal of this study was to develop and validate a noninvasive method to diagnose norovirus to complement stool diagnostics and to facilitate studies on transmission.MethodsA multiplex immunoassay to measure salivary immunoglobulin G (IgG) responses to 5 common norovirus genotypes (GI.1, GII.2, GII.4, GII.6, and GII.17) was developed. The assay was validated using acute and convalescent saliva samples collected from Peruvian children