학술논문
Increased Peripheral Blood Neutrophil Activation Phenotypes and Neutrophil Extracellular Trap Formation in Critically Ill Coronavirus Disease 2019 (COVID-19) Patients: A Case Series and Review of the Literature
Document Type
article
Author
Masso-Silva, Jorge A; Moshensky, Alexander; Lam, Michael TY; Odish, Mazen F; Patel, Arjun; Xu, Le; Hansen, Emily; Trescott, Samantha; Nguyen, Celina; Kim, Roy; Perofsky, Katherine; Perera, Samantha; Ma, Lauren; Pham, Josephine; Rolfsen, Mark; Olay, Jarod; Shin, John; Dan, Jennifer M; Abbott, Robert K; Ramirez, Sydney; Alexander, Thomas H; Lin, Grace Y; Fuentes, Ana Lucia; Advani, Ira; Gunge, Deepti; Pretorius, Victor; Malhotra, Atul; Sun, Xin; Duran, Jason; Hepokoski, Mark; Crotty, Shane; Coufal, Nicole G; Meier, Angela; Alexander, Laura E Crotty
Source
Clinical Infectious Diseases. 74(3)
Subject
Language
Abstract
BackgroundIncreased inflammation has been well defined in coronavirus disease 2019 (COVID-19), while definitive pathways driving severe forms of this disease remain uncertain. Neutrophils are known to contribute to immunopathology in infections, inflammatory diseases, and acute respiratory distress syndrome, a primary cause of morbidity and mortality in COVID-19. Changes in neutrophil function in COVID-19 may give insight into disease pathogenesis and identify therapeutic targets.MethodsBlood was obtained serially from critically ill COVID-19 patients for 11 days. Neutrophil extracellular trap formation (NETosis), oxidative burst, phagocytosis, and cytokine levels were assessed. Lung tissue was obtained immediately postmortem for immunostaining. PubMed searches for neutrophils, lung, and COVID-19 yielded 10 peer-reviewed research articles in English.ResultsElevations in neutrophil-associated cytokines interleukin 8 (IL-8) and interleukin 6, and general inflammatory cytokines IFN-inducible protien-19, granulocyte macrophage colony-stimulating factor (GM-CSF), interleukin 1β, interleukin 10, and tumor necrosis factor, were identified both at first measurement and across hospitalization (P