학술논문
Increased peripheral blood neutrophil activation phenotypes and NETosis in critically ill COVID-19 patients: a case series and review of the literature.
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article
Author
Masso-Silva, Jorge A; Moshensky, Alexander; Lam, Michael TY; Odish, Mazen; Patel, Arjun; Xu, Le; Hansen, Emily; Trescott, Samantha; Nguyen, Celina; Kim, Roy; Perofsky, Katherine; Perera, Samantha; Ma, Lauren; Pham, Josephine; Rolfsen, Mark; Olay, Jarod; Shin, John; Dan, Jennifer M; Abbott, Robert; Ramirez, Sydney; Alexander, Thomas H; Lin, Grace Y; Fuentes, Ana Lucia; Advani, Ira; Gunge, Deepti; Pretorius, Victor; Malhotra, Atul; Sun, Xin; Duran, Jason; Hepokoski, Mark; Crotty, Shane; Coufal, Nicole G; Meier, Angela; Alexander, Laura E Crotty
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Language
Abstract
BackgroundIncreased inflammation has been well defined in COVID-19, while definitive pathways driving severe forms of this disease remain uncertain. Neutrophils are known to contribute to immunopathology in infections, inflammatory diseases and acute respiratory distress syndrome (ARDS), a primary cause of morbidity and mortality in COVID-19. Changes in neutrophil function in COVID-19 may give insight into disease pathogenesis and identify therapeutic targets.MethodsBlood was obtained serially from critically ill COVID-19 patients for eleven days. Neutrophil extracellular trap formation (NETosis), oxidative burst, phagocytosis and cytokine levels were assessed. Lung tissue was obtained immediately post-mortem for immunostaining. Pubmed searches for neutrophils, lung and COVID-19 yielded ten peer-reviewed research articles in English.ResultsElevations in neutrophil-associated cytokines IL-8 and IL-6, and general inflammatory cytokines IP-10, GM-CSF, IL-1b, IL-10 and TNF, were identified both at first measurement and across hospitalization (p