학술논문

Genetic dissection of natural variation in oilseed traits of camelina by whole‐genome resequencing and QTL mapping
Document Type
article
Source
The Plant Genome. 14(2)
Subject
Agricultural
Veterinary and Food Sciences
Biological Sciences
Genetics
Agriculture
Land and Farm Management
Crop and Pasture Production
Horticultural Production
Biotechnology
Human Genome
Zero Hunger
Brassicaceae
Dissection
Genome-Wide Association Study
Plant Breeding
Quantitative Trait Loci
Plant Biology
Crop and pasture production
Plant biology
Language
Abstract
Camelina [Camelina sativa (L.) Crantz] is an oilseed crop in the Brassicaceae family that is currently being developed as a source of bioenergy and healthy fatty acids. To facilitate modern breeding efforts through marker-assisted selection and biotechnology, we evaluated genetic variation among a worldwide collection of 222 camelina accessions. We performed whole-genome resequencing to obtain single nucleotide polymorphism (SNP) markers and to analyze genomic diversity. We also conducted phenotypic field evaluations in two consecutive seasons for variations in key agronomic traits related to oilseed production such as seed size, oil content (OC), fatty acid composition, and flowering time. We determined the population structure of the camelina accessions using 161,301 SNPs. Further, we identified quantitative trait loci (QTL) and candidate genes controlling the above field-evaluated traits by genome-wide association studies (GWAS) complemented with linkage mapping using a recombinant inbred line (RIL) population. Characterization of the natural variation at the genome and phenotypic levels provides valuable resources to camelina genetic studies and crop improvement. The QTL and candidate genes should assist in breeding of advanced camelina varieties that can be integrated into the cropping systems for the production of high yield of oils of desired fatty acid composition.