학술논문
Comparative host-coronavirus protein interaction networks reveal pan-viral disease mechanisms
Document Type
article
Author
Gordon, David E; Hiatt, Joseph; Bouhaddou, Mehdi; Rezelj, Veronica V; Ulferts, Svenja; Braberg, Hannes; Jureka, Alexander S; Obernier, Kirsten; Guo, Jeffrey Z; Batra, Jyoti; Kaake, Robyn M; Weckstein, Andrew R; Owens, Tristan W; Gupta, Meghna; Pourmal, Sergei; Titus, Erron W; Cakir, Merve; Soucheray, Margaret; McGregor, Michael; Cakir, Zeynep; Jang, Gwendolyn; O’Meara, Matthew J; Tummino, Tia A; Zhang, Ziyang; Foussard, Helene; Rojc, Ajda; Zhou, Yuan; Kuchenov, Dmitry; Hüttenhain, Ruth; Xu, Jiewei; Eckhardt, Manon; Swaney, Danielle L; Fabius, Jacqueline M; Ummadi, Manisha; Tutuncuoglu, Beril; Rathore, Ujjwal; Modak, Maya; Haas, Paige; Haas, Kelsey M; Naing, Zun Zar Chi; Pulido, Ernst H; Shi, Ying; Barrio-Hernandez, Inigo; Memon, Danish; Petsalaki, Eirini; Dunham, Alistair; Marrero, Miguel Correa; Burke, David; Koh, Cassandra; Vallet, Thomas; Silvas, Jesus A; Azumaya, Caleigh M; Billesbølle, Christian; Brilot, Axel F; Campbell, Melody G; Diallo, Amy; Dickinson, Miles Sasha; Diwanji, Devan; Herrera, Nadia; Hoppe, Nick; Kratochvil, Huong T; Liu, Yanxin; Merz, Gregory E; Moritz, Michelle; Nguyen, Henry C; Nowotny, Carlos; Puchades, Cristina; Rizo, Alexandrea N; Schulze-Gahmen, Ursula; Smith, Amber M; Sun, Ming; Young, Iris D; Zhao, Jianhua; Asarnow, Daniel; Biel, Justin; Bowen, Alisa; Braxton, Julian R; Chen, Jen; Chio, Cynthia M; Chio, Un Seng; Deshpande, Ishan; Doan, Loan; Faust, Bryan; Flores, Sebastian; Jin, Mingliang; Kim, Kate; Lam, Victor L; Li, Fei; Li, Junrui; Li, Yen-Li; Li, Yang; Liu, Xi; Lo, Megan; Lopez, Kyle E; Melo, Arthur A; Moss, Frank R; Nguyen, Phuong; Paulino, Joana; Pawar, Komal Ishwar; Peters, Jessica K
Source
Science. 370(6521)
Subject
Language
Abstract
The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is a grave threat to public health and the global economy. SARS-CoV-2 is closely related to the more lethal but less transmissible coronaviruses SARS-CoV-1 and Middle East respiratory syndrome coronavirus (MERS-CoV). Here, we have carried out comparative viral-human protein-protein interaction and viral protein localization analyses for all three viruses. Subsequent functional genetic screening identified host factors that functionally impinge on coronavirus proliferation, including Tom70, a mitochondrial chaperone protein that interacts with both SARS-CoV-1 and SARS-CoV-2 ORF9b, an interaction we structurally characterized using cryo-electron microscopy. Combining genetically validated host factors with both COVID-19 patient genetic data and medical billing records identified molecular mechanisms and potential drug treatments that merit further molecular and clinical study.