학술논문
c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae.
Document Type
article
Author
Floyd, Kyle A; Lee, Calvin K; Xian, Wujing; Nametalla, Mahmoud; Valentine, Aneesa; Crair, Benjamin; Zhu, Shiwei; Hughes, Hannah Q; Chlebek, Jennifer L; Wu, Daniel C; Hwan Park, Jin; Farhat, Ali M; Lomba, Charles J; Ellison, Courtney K; Brun, Yves V; Campos-Gomez, Javier; Dalia, Ankur B; Liu, Jun; Biais, Nicolas; Wong, Gerard CL; Yildiz, Fitnat H
Source
Nature communications. 11(1)
Subject
Language
Abstract
Biofilm formation by Vibrio cholerae facilitates environmental persistence, and hyperinfectivity within the host. Biofilm formation is regulated by 3',5'-cyclic diguanylate (c-di-GMP) and requires production of the type IV mannose-sensitive hemagglutinin (MSHA) pilus. Here, we show that the MSHA pilus is a dynamic extendable and retractable system, and its activity is directly controlled by c-di-GMP. The interaction between c-di-GMP and the ATPase MshE promotes pilus extension, whereas low levels of c-di-GMP correlate with enhanced retraction. Loss of retraction facilitated by the ATPase PilT increases near-surface roaming motility, and impairs initial surface attachment. However, prolonged retraction upon surface attachment results in reduced MSHA-mediated surface anchoring and increased levels of detachment. Our results indicate that c-di-GMP directly controls MshE activity, thus regulating MSHA pilus extension and retraction dynamics, and modulating V. cholerae surface attachment and colonization.