학술논문

A highly contiguous reference genome for the Steller’s jay (Cyanocitta stelleri)
Document Type
article
Source
Journal of Heredity. 114(5)
Subject
Biological Sciences
Bioinformatics and Computational Biology
Genetics
Animals
Genome
Passeriformes
Genomics
Base Sequence
Chromosomes
Sex Chromosomes
California Conservation Genomics Project
Corvidae
transposable elements
Evolutionary Biology
Evolutionary biology
Language
Abstract
The Steller's jay is a familiar bird of western forests from Alaska south to Nicaragua. Here, we report a draft reference assembly for the species generated from PacBio HiFi long-read and Omni-C chromatin-proximity sequencing data as part of the California Conservation Genomics Project (CCGP). Sequenced reads were assembled into 352 scaffolds totaling 1.16 Gb in length. Assembly metrics indicate a highly contiguous and complete assembly with a contig N50 of 7.8 Mb, scaffold N50 of 25.8 Mb, and BUSCO completeness score of 97.2%. Repetitive elements span 16.6% of the genome including nearly 90% of the W chromosome. Compared with high-quality assemblies from other members of the family Corvidae, the Steller's jay genome contains a larger proportion of repetitive elements than 4 crow species (Corvus), but a lower proportion of repetitive elements than the California scrub-jay (Aphelocoma californica). This reference genome will serve as an essential resource for future studies on speciation, local adaptation, phylogeography, and conservation genetics in this species of significant biological interest.