학술논문

A highly contiguous genome assembly for the pocket mouse Perognathus longimembris longimembris
Document Type
article
Source
Journal of Heredity. 115(1)
Subject
Biological Sciences
Ecology
Genetics
Human Genome
Life on Land
Animals
Mice
Genome
Chromosomes
Genomics
North America
California Conservation Genomics Project
comparative genomics
conservation genetics
Heteromyidae
Perognathus
repetitive elements
Evolutionary Biology
Evolutionary biology
Language
Abstract
The little pocket mouse, Perognathus longimembris, and its nine congeners are small heteromyid rodents found in arid and seasonally arid regions of Western North America. The genus is characterized by behavioral and physiological adaptations to dry and often harsh environments, including nocturnality, seasonal torpor, food caching, enhanced osmoregulation, and a well-developed sense of hearing. Here we present a genome assembly of Perognathus longimembris longimembris generated from PacBio HiFi long read and Omni-C chromatin-proximity sequencing as part of the California Conservation Genomics Project. The assembly has a length of 2.35 Gb, contig N50 of 11.6 Mb, scaffold N50 of 73.2 Mb, and includes 93.8% of the BUSCO Glires genes. Interspersed repetitive elements constitute 41.2% of the genome. A comparison with the highly endangered Pacific pocket mouse, P. l. pacificus, reveals broad synteny. These new resources will enable studies of local adaptation, genetic diversity, and conservation of threatened taxa.