부산대학교 도서관

UnlabelledWe report on a radiopharmaceutical imaging platform designed to capture the kinetics of cellular responses to drugs.MethodsA portable in vitro molecular imaging system comprising a microchip and a β-particle imaging camera permitted routine cell-based radioassays of small numbers of either suspended or adherent cells. We investigated the kinetics of responses of model lymphoma and glioblastoma cancer cell lines to (18)F-FDG uptake after drug exposure. Those responses were correlated with kinetic changes in the cell cycle or with changes in receptor tyrosine kinase signaling.ResultsThe platform enabled direct radioassays of multiple cell types and yielded results comparable to those from conventional approaches; however, the platform used smaller sample sizes, permitted a higher level of quantitation, and did not require cell lysis.ConclusionThe kinetic analysis enabled by the platform provided a rapid (≈ 1 h) drug screening assay.