학술논문
Precision and accuracy of single-molecule FRET measurements—a multi-laboratory benchmark study
Document Type
article
Author
Hellenkamp, Björn; Schmid, Sonja; Doroshenko, Olga; Opanasyuk, Oleg; Kühnemuth, Ralf; Rezaei Adariani, Soheila; Ambrose, Benjamin; Aznauryan, Mikayel; Barth, Anders; Birkedal, Victoria; Bowen, Mark E; Chen, Hongtao; Cordes, Thorben; Eilert, Tobias; Fijen, Carel; Gebhardt, Christian; Götz, Markus; Gouridis, Giorgos; Gratton, Enrico; Ha, Taekjip; Hao, Pengyu; Hanke, Christian A; Hartmann, Andreas; Hendrix, Jelle; Hildebrandt, Lasse L; Hirschfeld, Verena; Hohlbein, Johannes; Hua, Boyang; Hübner, Christian G; Kallis, Eleni; Kapanidis, Achillefs N; Kim, Jae-Yeol; Krainer, Georg; Lamb, Don C; Lee, Nam Ki; Lemke, Edward A; Levesque, Brié; Levitus, Marcia; McCann, James J; Naredi-Rainer, Nikolaus; Nettels, Daniel; Ngo, Thuy; Qiu, Ruoyi; Robb, Nicole C; Röcker, Carlheinz; Sanabria, Hugo; Schlierf, Michael; Schröder, Tim; Schuler, Benjamin; Seidel, Henning; Streit, Lisa; Thurn, Johann; Tinnefeld, Philip; Tyagi, Swati; Vandenberk, Niels; Vera, Andrés Manuel; Weninger, Keith R; Wünsch, Bettina; Yanez-Orozco, Inna S; Michaelis, Jens; Seidel, Claus AM; Craggs, Timothy D; Hugel, Thorsten
Source
Nature Methods. 15(9)
Subject
Language
Abstract
Single-molecule Förster resonance energy transfer (smFRET) is increasingly being used to determine distances, structures, and dynamics of biomolecules in vitro and in vivo. However, generalized protocols and FRET standards to ensure the reproducibility and accuracy of measurements of FRET efficiencies are currently lacking. Here we report the results of a comparative blind study in which 20 labs determined the FRET efficiencies (E) of several dye-labeled DNA duplexes. Using a unified, straightforward method, we obtained FRET efficiencies with s.d. between ±0.02 and ±0.05. We suggest experimental and computational procedures for converting FRET efficiencies into accurate distances, and discuss potential uncertainties in the experiment and the modeling. Our quantitative assessment of the reproducibility of intensity-based smFRET measurements and a unified correction procedure represents an important step toward the validation of distance networks, with the ultimate aim of achieving reliable structural models of biomolecular systems by smFRET-based hybrid methods.