학술논문
Best practices and benchmarks for intact protein analysis for top-down mass spectrometry
Document Type
article
Author
Donnelly, Daniel P; Rawlins, Catherine M; DeHart, Caroline J; Fornelli, Luca; Schachner, Luis F; Lin, Ziqing; Lippens, Jennifer L; Aluri, Krishna C; Sarin, Richa; Chen, Bifan; Lantz, Carter; Jung, Wonhyeuk; Johnson, Kendall R; Koller, Antonius; Wolff, Jeremy J; Campuzano, Iain DG; Auclair, Jared R; Ivanov, Alexander R; Whitelegge, Julian P; Paša-Tolić, Ljiljana; Chamot-Rooke, Julia; Danis, Paul O; Smith, Lloyd M; Tsybin, Yury O; Loo, Joseph A; Ge, Ying; Kelleher, Neil L; Agar, Jeffrey N
Source
Nature Methods. 16(7)
Subject
Language
Abstract
One gene can give rise to many functionally distinct proteoforms, each of which has a characteristic molecular mass. Top-down mass spectrometry enables the analysis of intact proteins and proteoforms. Here members of the Consortium for Top-Down Proteomics provide a decision tree that guides researchers to robust protocols for mass analysis of intact proteins (antibodies, membrane proteins and others) from mixtures of varying complexity. We also present cross-platform analytical benchmarks using a protein standard sample, to allow users to gauge their proficiency.