학술논문
Identification of Luteovirus Nucleotide Sequences in Mild Yellow-Edge Diseased Strawberry Plants
Document Type
Academic Journal
Source
The Plant Pathology Journal. 2002-02 18(1):1-5
Subject
Language
Korean
ISSN
1598-2254
2093-9280
2093-9280
Abstract
The availability of nucleotide sequences of the coat protein gene of Potato leafroll virus (PLRV) permitted the construction of DNA primers that were utilized for cDNA synthesis. Polymerase chain reaction (PCR) products of a 487 bp. and approximately 500 bp DNA fragments were amplified from nucleic acid extracts of PLRV-infected tissue and strawberry mild yellow-edge (SMYE) diseased strawberry tissue, respectively. The amplified DNA fragments were further differentiated by hybridization analysis with a cDNA probe for the coat protein gene of PLRV and restriction fragment length polymorphism (RFLP) analysis. These results suggest that a luteovirus is associated with the SMYE disease.