부산대학교 도서관

This study was performed to determine the whitening effect of several solvent fractions of Scolopedra subspinipes mutilans(SSM) extracts. Fractions from ethanol and distilled water extracts of SSM were prepared by a systematic fractionation procedure using n-hexane, chloroform, ethyl acetate, n-butanol and d.w. Also, Cholroform, ethyl acetate and n-hexane extractions of SSM were analyzed by gas chromatography-mass spectrometry (GC-MS). The results demonstrated that 4 components were identified totally in samples, and 4-Hydroxyphenyl acetic acid(4-HPAA) was confirmed as a selective component for whitening effect and melanin synthesis in B16F10 melanoma cells. The 4-HPAA showed the tyrosinase inhibition activity to 77.34% and concentration-dependent. These results showed that fractions from SSM and 4-HPAA could be developed as a skin whitening material in cosmetics.