학술논문
Upregulation of Membrane-Bound CD40L on CD4+ T cells in Women with Primary Sjögrenʼs Syndrome
Document Type
Academic Journal
Author
Source
Scandinavian Journal of Immunology. Jan 01, 2014 79(1):37-42
Subject
Language
English
ISSN
0300-9475
Abstract
ABSTRACT: Epigenetic deregulation of genes encoded on the X chromosome as reported for CD40L in lupus could explain the female predominance of autoimmune diseases. We compared CD40L expression on CD4 T cells from primary Sjögrenʼs syndrome (pSS) women and healthy controls and investigated DNA methylation patterns of the promoter and enhancer regions of CD40L. The expression of CD40L on activated CD4 T cells was higher in patients with pSS than controls after phorbolmyristate acetate and ionomycin activation (P = 0.02). CD40L mRNA level in CD4 T cells did not differ between patients with pSS and controls and was similar in both groups in cultures treated with the demethylating agent 5-azacytidine C. Pyrosequencing analysis revealed no significant differences in methylation profiles between patients and controls. Inducible membrane-bound CD40L on CD4 T cells is increased in patients with pSS but was not related to epigenetic deregulation by demethylation patterns of the regulatory regions of CD40L.