부산대학교 도서관

OBJECTIVE: The aim of our study was to evaluate the influence of smoking on the activity of N-acetyl-β-hexosaminidase (HEX), its isoenzymes A (HEX-A) and B (HEX-B) and β-galactosidase (GAL), in the saliva of patients with Type 1 diabetes. METHODS: In the supernatant HEX and its isoenzymes A and B, and β-galactosidase were determined by the method of Chatteriee et al in modification of Zwierz et al (mKat kg of protein). Protein was determined by the Lowry et al method (mg ml). RESULTS: The results presented here suggest that diabetes and smoking modify activity of HEX and its isoenzymes, but only combination of diabetes and smoking give a significant increase in the specific activity of HEX and its isoenzymes. CONCLUSIONS: Type 1 diabetes slightly changes the composition of saliva. Smoking cigarettes significantly modifies the composition and properties of saliva in healthy individuals and patients with Type 1 diabetes.