학술논문
Biological characteristics of myelodysplastic syndrome patients who demonstrated high versus no intramedullary apoptosis
Document Type
Academic Journal
Author
Dar, Saleem; Mundle, Suneel; Andric, Tanja; Qawi, Huma; Shetty, Vilasini; Reza, Samina; Mativi, B. Yifwayimare; Allampallam, Krishnan; Ali, Ambereen; Venugopal, Parameswaren; Gezer, Sefer; Broady-Robinson, LaTanya; Cartlidge, John; Showel, Margaret; Hussaini, Seema; Ragasa, Deborah; Ali, Irfan; Chaudhry, Ambreen; Waggoner, Samina; Lisak, Laurie; Huang, Ray-Win; Raza, Azra
Source
European Journal of Haematology. Feb 01, 1999 62(2):90-94
Subject
Language
English
ISSN
0902-4441
Abstract
Spontaneous intramedullary apoptosis was measured in bone marrow (BM) biopsies of 175 patients with myelodysplastic syndromes (MDS) using in situ end-labeling (ISEL) of fragmented DNA. Two groups of high (n = 71) versus low (n = 43) levels of apoptosis were identified while 61 patients were ISEL-negative. Semiquantitative assessment of 3 cytokines, the number of macrophages and in vivo labeling indices (LI) were also determined from consecutive sections of the biopsy. Patients with high apoptosis levels tended to have a high LI (p = 0.013), more macrophages in their BM biopsies (p = 0.006) and higher tumor necrosis factor alpha (TNF-α) levels (not significant) compared to patients with no apoptosis. In addition, low risk MDS patients had significantly lower rates of apoptosis (p = 0.047) and lower levels of TNF-α (p = 0.055) compared to high-risk MDS patients. We conclude that the genesis of cytopenias in MDS is of multifactorial origin and that cytokine-associated apoptosis clearly identifies a distinct biological subgroup of patients who may benefit selectively by use of anti-cytokine therapies.