학술논문
Expression of Bacillus subtilis phytase in Lactobacillus plantarum 755
Document Type
Academic Journal
Author
Source
Letters in Applied Microbiology. Apr 01, 2000 30(4):325-329
Subject
Language
English
ISSN
0266-8254
Abstract
Phytase enzymes can increase the nutritional value of food and feed by liberating inorganic phosphate from phytate, the major storage form of phosphorus in plants. The phytase (phyC) from Bacillus subtilis VTT E-68013 was expressed in Lactobacillus plantarum strain 755 using Lact. amylovorus α-amylase secretion signals. In an overnight cultivation in MRS medium containing cellobiose for induction of the α-amylase promoter, catalytically active phytase was secreted as a predominant extracellular protein. However, Western blot analysis revealed unprocessed and processed phytase in the cell fraction. Pulse chase experiments showed that the recombinant phytase was secreted at a slower rate in comparison to the native proteins of Lact. plantarum 755.