학술논문
Defective DNA double-strand break repair in pediatric systemic lupus erythematosus
Document Type
Academic Journal
Author
Source
Arthritis & Rheumatism. Feb 01, 2012 64(2):568-578
Subject
Language
English
ISSN
0004-3591
Abstract
OBJECTIVE.: METHODS.: Nine assays were used to interrogate DSB repair and recognition in lymphoblastoid cell lines from patients with pediatric SLE, including the neutral comet assay (NCA), colony survival assay (CSA), irradiation-induced foci formation for γ-H2AX and 53BP1 proteins, kinetics of phosphorylation of structural maintenance of chromosomes protein 1 (SMC1), postirradiation bromodeoxyuridine incorporation to evaluate S phase checkpoint integrity, monoubiquitination of Fanconi protein D2, ATM protein expression, and non-homologous DNA end joining protein expression and function. RESULTS.: CONCLUSION.