학술논문
Flow Cytometry Characterization of Cerebrospinal Fluid Monocytes in Patients With Postoperative Cognitive Dysfunction: A Pilot Study
Document Type
Academic Journal
Author
Berger, Miles; Murdoch, David M.; Staats, Janet S.; Chan, Cliburn; Thomas, Jake P.; Garrigues, Grant E.; Browndyke, Jeffrey N.; Cooter, Mary; Quinones, Quintin J.; Mathew, Joseph P.; Weinhold, Kent J.; Amundsen, Cindy L.; Bengali, Shahrukh; Brigman, Brian E.; Bullock, W. Michael; Carter, Jessica; Chapman, Joseph; Cheong Yee Ching, Vanessa; Cohen, Harvey J.; Colin, Brian; D’Amico, Thomas A.; Devinney, Michael J.; DeOrio, James K.; Ellet, Tressa; Esclamado, Ramon M.; Ferrandino, Michael N.; Gadsden, Jeffrey; Guercio, Jason; Habib, Ashraf; Harpole, David H.; Hartwig, Mathew G.; Iboaya, Ehimemen; Inman, Brant A.; Khan, Anver; Lagoo-Deenadayalan, Sandhya; Lee, Paula S.; Lee, Walter T.; Lemm, John; Levinson, Howard; Mantyh, Christopher; McDonagh, David L.; Migaly, John; Mithani, Suhail K.; Moretti, Eugene; Moul, Judd W.; Newman, Mark F.; Ni, Katherine; Ohlendorf, Brian; Perez, Alexander; Peterson, Andrew C.; Ponussamy, Vikram; Preminger, Glenn M.; Robertson, Cary N.; Roman, Sanziana A.; Runyon, Scott; Sandler, Aaron; Scheri, Randall P.; Smith, S. Kendall; Talbot, Leonard; Thacker, Julie K. M.; Tong, Betty C.; Tu, Alexander; Vaslef, Steven N.; Waldron, Nathan; Wang, Xueyuan; Whitson, Heather; Wickenheisser, Victoria; Young, Christopher
Source
Anesthesia & Analgesia. Nov 01, 2019 129(5):e150-e154
Subject
Language
English
ISSN
0003-2999
Abstract
Animal models suggest postoperative cognitive dysfunction may be caused by brain monocyte influx. To study this in humans, we developed a flow cytometry panel to profile cerebrospinal fluid (CSF) samples collected before and after major noncardiac surgery in 5 patients ≥60 years of age who developed postoperative cognitive dysfunction and 5 matched controls who did not. We detected 12,654 ± 4895 cells/10 mL of CSF sample (mean ± SD). Patients who developed postoperative cognitive dysfunction showed an increased CSF monocyte/lymphocyte ratio and monocyte chemoattractant protein 1 receptor downregulation on CSF monocytes 24 hours after surgery. These pilot data demonstrate that CSF flow cytometry can be used to study mechanisms of postoperative neurocognitive dysfunction.