학술논문
Identification of an I2 Binding Protein From Rabbit Brain
Document Type
Academic Journal
Author
Source
Annals of the New York Academy of Sciences. Jan 01, 2003 1009:364-366
Subject
Language
English
ISSN
0077-8923
Abstract
Imidazoline-2 binding proteins exist as a heterogeneous population. The aim of this study was to isolate and identify I2 binding proteins from rabbit brain using an affinity column synthesized with a highly selective I2 ligand, 2-(2-benzofuranyl)2-imidazoline (2BFI). The results revealed an approximately 45-kD protein to be brain creatine kinase (EC 2.7.3.2). [H]-2BFI (5nM) was able to bind specifically to the purified enzyme. This study has identified brain creatine kinase as a novel I2 binding protein.