학술논문
Reduced expression of the adhesion protein tensin1 in cultured human dermal fibroblasts affects collagen gel contraction
Document Type
Academic Journal
Author
Source
Experimental Dermatology. Sep 01, 2008 17(9):788-789
Subject
Language
English
ISSN
0906-6705
Abstract
As revealed by immunohistochemistry and RT-QPCR, the focal adhesion protein tensin1 is expressed in cultured human dermal fibroblasts and reduced by 60% after transfection with tensin1 siRNA. Tensin1 silenced fibroblast exhibited a strongly reduced capacity to contract collagen gels. Aged fibroblasts, generated with the Hayflick replicative senescence model, exhibit as siRNA silences fibroblasts, a reduced tensin1 expression and an impaired gel contraction capacity. Based on these results, we speculate that in human dermal fibroblasts, tensin1 plays an important role in cell–matrix interaction and that a reduced expression might contribute to the dermal alterations observed during skin ageing.