부산대학교 도서관

The Check-MDR CT102 DNA microarray enables detection of the most prevalent carbapenemases (NDM, VIM, KPC, OXA-48 and IMP) and extended-spectrum β-lactamase (ESBL) gene families (SHV, TEM and CTX-M). The test performance of this microarray was evaluated with 95 Enterobacteriaceae isolates suspected of being carbapenemase producers, i.e. with meropenem MICs ⋝0.5 mg l. The collection of isolates contained 70 carbapenemase-producing isolates, including 37 blaKPC-, 20 blaVIM-, five blaOXA-48-, four blaKPC/blaVIM- and four blaNDM-positive isolates; and 25 carbapenemase-gene-negative isolates. ESBLs were produced by 51 of the isolates. PCR and sequencing of β-lactamase genes was used as reference test. For detection of carbapenemases, the sensitivity of the microarray was 97 % (68/70), with 100 % specificity. The two negative isolates tested positive when the microarray test was repeated; these isolates were an OXA-48- and a KPC-producing isolate. For ESBL detection, the sensitivity was 100 % (51/51) and the specificity was 98 % (43/44), although 20 % of the SHV-12 ESBLs were categorized as SHV-2-like ESBLs. In conclusion, the CDT102 microarray is a rapid and accurate tool for the detection of carbapenemase and ESBL genes, although the array seems less suitable for epidemiology of ESBL genes.