부산대학교 도서관

BACKGROUND.: The human prostate tumor suppressor NKX3.1 mediates the DNA repair response and interacts with the androgen receptor to assure faithful completion of transcription thereby protecting against TMPRSS2-ERG gene fusion. To determine directly the effect of Nkx3.1 in vivo we studied the DNA repair response in prostates of mice with targeted deletion of Nkx3.1. METHODS.: Using both drug-induced DNA damage and γ-irradiation, we assayed expression of γ-histone 2AX at time points up to 24 hr after induction of DNA damage. RESULTS.: We demonstrated that expression of Nkx3.1 influenced both the timing and magnitude of the DNA damage response in the prostate. CONCLUSIONS.: Nkx3.1 affects the DNA damage response in the murine prostate and is haploinsufficient for this phenotype. Prostate 76:402–408, 2016. © 2015 The Authors. The Prostate published by Wiley Periodicals, Inc.