학술논문
Cyclin E low molecular weight isoforms occur commonly in early-onset gastric cancer and independently predict survival
Document Type
Academic Journal
Author
Source
Journal of Clinical Pathology. Mar 01, 2008 61(3):311-316
Subject
Language
English
ISSN
0021-9746
Abstract
BACKGROUND:: Post-translational cleavage of full-length cyclin E from the N-terminus can produce low molecular weight (LMW) isoforms of cyclin E containing the C-terminus only. AIM:: To assess their presence in early-onset gastric cancer (EOGC), stump cancers and conventional gastric cancers and ascertain how they influence survival in EOGC. METHODS:: The expression of full-length and LMW isoforms of cyclin E in 330 gastric cancers, including early-onset gastric cancer (EOGC), stump cancer and conventional gastric cancer (>45 years old) was compared using antibodies targeted to the N- and C-terminals. RESULTS:: LMW isoforms were found in 35% of EOGCs, compared to 8% of conventional gastric cancers and 4% of stump cancers; their presence was visualised in cell lines using western blot analysis. In addition, C-terminal staining was a positive predictor of survival in EOGC. In contrast, no correlation with survival was found with the N-terminal antibody which detects only full-length cyclin E. CONCLUSION:: EOGCs have a unique molecular phenotype and LMW isoforms of cyclin E may independently influence survival in EOGC.