부산대학교 도서관

An efficient liquid culture system for plant regeneration from leafless stem–root axes of Cryptanthus sinuosus L. B. Smith (Bromeliaceae) was established. High regeneration rates (93%) were achieved in Murashige and Skoogʼs medium without growth regulators. Whole plants were obtained in a single-step procedure, resulting in the production of 25.3 ± 3.6 plants/explant after 6 months of culture. Incubation of plant material at 35 ± 3 °C resulted in an increase of 60% in the regeneration efficiency compared with tissues incubated at 28 ± 2 °C. Moreover, after 5–6 sub-cultures in the same medium, the axes originated bud clusters that could be continuously multiplied and gave rise to 19.4 ± 3.2 whole plants per gram of fresh matter. It was estimated that the liquid culture system described is potentially able to produce about 4500 plants/explant/year. Rates of 98% acclimatization were achieved. The use of plants produced following this method for population reinforcement and for in vitro preservation programs of endangered populations is suggested.