학술논문
Characterization of plasmids carrying the blaOXA-24/40 carbapenemase gene and the genes encoding the AbkA/AbkB proteins of a toxin/antitoxin system
Document Type
Academic Journal
Author
Mosqueda, Noraida; Gato, Eva; Roca, Ignasi; López, María; de Alegría, Carlos Ruíz; Fernández Cuenca, Felipe; Martínez-Martínez, Luis; Pachón, Jerónimo; Cisneros, José Miguel; Rodríguez-Baño, Jesús; Pascual, Álvaro; Vila, Jordi; Bou, Germán; Tomás, María; Garnacho, José; Pizarraya, Antonio Gutierrez; Márquez Vácaro, Juan Antonio; Cano, María Eliecer; Fariñas, M. Carmen; Porto, Antonio Sánchez; Meruendano, Gloria Esteban; Vales, Luis Barbeyto; Ciria, Javier Casas; Vallejo, Luis; Pérez, Begona Fernández; Chao, José Carlos Villar; Ortega, Belén Padilla; Mansilla, Emilia Cercenado; Irure, José Javier García; del Arco Jiménez, Alfonso; Cardona, Concepción Gimeno; Valía, Juan Carlos; Palop, Núria Tormo; Abril, Vicente; Rifa, Josefina; Jesus, Maria; Garcia, Martinez; Pujals, Joseph Vilaró; Aguirre, Marian Navarro; Vilamala, Ana; Alfaro, José Antonio Jiménez; Jaca, Carlos Reviejo; Casanova, Pilar Marín; Guerreo, Francisca; Shaw, Evelyn; Plasencia, Virginia; Mayoral, Teresa Nebreda; Calavia, María José Fernández; de Cruz, Susana García; Mansilla, Carmen Aldea; de Lucas, Esperanza Merino; Zorraquino, Alfredo; Bañuls, Sergio Reus; Eseverri, Eugenio Garduno; Sánchez, Luis López; Gutiérrez, Ana Fleites; Guardado, Azucena Rodríguez; Moreno, Alfonso; Anguera, José María García-Arenzana; Palmero, Serafín López; Maresca, Manuel Rodríguez; Garrote, Fernando García; Otero, José Varela; del Pilar Alonso, María; Verdú, Elisa Vidal; López, Fernando Rodríguez; Sánchez, Fernanda Pardo; Vizoso, E. Ferrer; Garcia, B. Regueiro; Gurgui, Mercé; Pericas, Roser; Pomar, Virginia; Astigarraga, Pedro María Olaechea; Igartua, Rafael Ayarza; Romero, María Dolores Maciá; de Gopegui Bordes, Enrique Ruiz; Romero, María Isabel Sánchez; Mata, Jesús García; Goyanes, María José; Mateos, Cristina Morales; Quero, José Hernández; Lara, Trinidad Escobar; Bastardie, Frederic Ballester; Iftimie, Simona; Bajador, Isabel Pujol; Navarro, María Isabel Galán; Gurrea, María Luz Cádiz; Antequera, Carmen Amores; Gómez, Montserrat; Cantudo, Purificación; Salas, Carmina Martí; Peragosa, Jordi Cuquet; Flores, Antonio Moreno; García, Luis Anibarro; Real, Susana Hernando; González, Pablo A. Carrero; González, María Angeles Pallarés; Fernández, Sergio Rodríguez; Rojo, Miquel Pujol; Tubau, Fe; Alvarez, Enrique Nuno; Torres, María Ortega; Almaraz, Salvador Giner; Castelló, María Rosa Roca; Castillo, Manuela; Hortelano, Elena; Sánchez, Fernando Chaves; Reyne, Ana García; Gallego, Juan Pablo Horcajada; Segura, Concha; Dorado, Gema Sierra; Escudero, Raquel Yano; Hung, María Elena Dorta; del Rosario Q, Cristóbal
Source
Journal of Antimicrobial Chemotherapy. Oct 01, 2014 69(10):2629-2633
Subject
Language
English
ISSN
0305-7453
Abstract
BACKGROUND: Carbapenem-resistant Acinetobacter baumannii (CRAb) is a major source of nosocomial infections in Spain associated with the production of OXA-58-like or OXA-24/40-like β-lactamase enzymes. We analysed the plasmids carrying the blaOXA-24/40-like gene in CRAb isolates obtained a decade apart. METHODS: The presence of β-lactamases was screened for by PCR (metallo-β-lactamases, carbapenem-hydrolysing class D β-lactamases, GES and KPC) in 101 CRAb isolates obtained in two multicentre studies (GEIH/REIPI-Ab-2000 and GEIH/REIPI-Ab-2010; n = 493 Acinetobacter spp). We analysed the distribution and characterization of the plasmids carrying the blaOXA-24/40-like gene and sequenced two plasmids, AbATCC223p (2000) and AbATCC329p (2010) from A. baumannii ATCC 17978 transformants. RESULTS: Acquisition of the blaOXA-24/40-like gene was the main mechanism underlying resistance to carbapenems (48.7% in 2000 compared with 51.6% in 2010). This gene was mainly isolated in ST2 A. baumannii strains in both studies, although some novel STs (ST79 and ST80) appeared in 2010. The gene was located in plasmids (8–12 kbp) associated with the repAci2 or repAci2/repGR12 types. The sequences of AbATCC223p (8840 bp) and AbATCC329p (8842 bp) plasmids were similar, particularly regarding the presence of the genes encoding the AbkA/AbkB proteins associated with the toxin/antitoxin system. Moreover, the abkA/abkB gene sequences (>96% identity) were also located in plasmids harbouring the blaOXA-58-like gene. CONCLUSIONS: The action of OXA-24/40 and OXA-58 β-lactamase-like enzymes represents the main mechanism underlying resistance to carbapenems in Spain in the last decade. AbkA/AbkB proteins in the toxin/antitoxin system may be involved in the successful dissemination of plasmids carrying the blaOXA-24/40-like gene, and probably also the blaOXA-58-like gene, thus contributing to the plasmid stability.