학술논문
Análisis preliminar del papel de Rho1p en el citoesqueleto de actina en Schizosaccharomyces pombe
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[ES] Las GTPasas de la familia Rho son proteínas clave en la organización del crecimiento, regulan el citoesqueleto de actina y están conservadas evolutivamente. Estas proteínas juegan un papel muy importante no solo en el crecimiento de las levaduras sino también en procesos como el desarrollo neuronal o el cáncer. En S. pombe, se sabe que la GTPasa Rho1p participa en la síntesis de la pared celular, pero se sabe muy poco de su función reguladora del citoesqueleto de actina. Se ha visto que la depleción de Rho1 va acompañada de la desaparición de la actina polimerizada y su incremento produce cúmulos de actina no polarizada. En este trabajo, hemos sobreexpresado Rho1 y sus GEFs en una serie de mutantes de proteínas de unión a actina que regulan la nucleación, la estabilidad, el intercambio de nucleósido en los monómeros, etc con el propósito de detectar posibles candidatos que puedan interaccionar con Rho1. Hemos visto que la sobreexpresión de Rho1 mejora el crecimiento en los mutantes de la formina Cdc12, de la tropomiosina Cdc8 y de la miosina Cdc4, todos ellos involucrados en la formación y estabilización del anillo de actomiosina.
[EN] Rho GTPases are conserved molecules that control cytoskeletal dynamics. These proteins also play very important roles in processes such as neuronal development or cancer progression. Fission yeast Rho1 has been involved in cell wall biosynthesis, however little is known about its regulatory function of the actin cytoskeleton. In this sense, it has been shown that Rho1 depletion is accompanied by the disappearance of the polymerized actin, while an increase in Rho1 produces clusters of unpolarized actin. In this work, we have overexpressed Rho1 and its GEFs (Rgf1, Rgf2 and Rgf3) in a series of mutants of actin-binding protein which regulate nucleation, stability, monomers nucleoside exchange, etc. in order to detect potential candidates that can interact with Rho1. We saw that Rho1 overexpression improves the growth of a formin mutant (cdc12), a tropomyosin mutant (cdc8) and a myosin mutant (cdc4). All of them are involved in the formation and the stabilization of the actomyosin ring
[EN] Rho GTPases are conserved molecules that control cytoskeletal dynamics. These proteins also play very important roles in processes such as neuronal development or cancer progression. Fission yeast Rho1 has been involved in cell wall biosynthesis, however little is known about its regulatory function of the actin cytoskeleton. In this sense, it has been shown that Rho1 depletion is accompanied by the disappearance of the polymerized actin, while an increase in Rho1 produces clusters of unpolarized actin. In this work, we have overexpressed Rho1 and its GEFs (Rgf1, Rgf2 and Rgf3) in a series of mutants of actin-binding protein which regulate nucleation, stability, monomers nucleoside exchange, etc. in order to detect potential candidates that can interact with Rho1. We saw that Rho1 overexpression improves the growth of a formin mutant (cdc12), a tropomyosin mutant (cdc8) and a myosin mutant (cdc4). All of them are involved in the formation and the stabilization of the actomyosin ring