부산대학교 도서관

Objective: This study was conducted to investigate the effect of L-glutathione supplementation on oxidative stress (OS) markers and antioxidant gene expression in C57BL/6NTac diabetic mice. Methods: Twenty-four male C57BL/6NTac mice, 16 weeks of age, were divided into four groups; C, G, D, and DGSH (n=6). Diabetic mice (Groups D and DGSH) were injected with streptozotocin (STZ) at 50 mg / kg bw in 0.1 ml of Na-Citrate buffer. Control mice (Groups C and G) were injected with an equal dose of Na-Citrate buffer. L-glutathione at 15 mg/kg bw was intraperitoneally administered to the Group DGSH mice weekly. Testes were collected for evaluation after 42 days of treatment. To assess oxidative damage, serum and testicular tissue malondialdehyde (MDA) and 8-hydroxy deoxyguanosine (8-OHdG) were measured. The mRNA expression of antioxidant enzymes, superoxide dismutase 1 (Sod1) and glutathione peroxidase 1 (Gpx-1) in the testes were quantified using a real time-quantitative polymerase chain reaction (RT-qPCR). Data were then analysed using one-way ANOVA. Results: Significant decreased of testicular MDA and 8-OHdG were observed in Group DGSH mice compared to the Group D (p<0.01). However, significant elevation of serum MDA was observed in Group DGSH mice, relative to the mice in the Group C. While for mRNA expression, Sod1 and Gpx-1 were upregulated in Group DGSH when compared to Groups D and G, respectively. Conclusion: Conclusion: Oxidative stress (OS) state in testes were confirmed by the elevated levels of MDA and 8-OHdG in Group D. Enhanced Sod1 and Gpx-1 expression in Group DGSH showed that L-glutathione at 15 mg/kg bw was able to minimize the harmful effects of diabetes. Present data indicated the existence of several processes that contribute to, and possibly interact with, impaired testicular function in diabetic mice, some of which are potentially susceptible to therapeutic interventions with L-glutathione. [Funding: Ministry of Higher Education Malaysia FRGS 5/3 (273/2019)]