학술논문
17-베타 에스트라디올이 OLETF 쥐의 지방세포 Lipin-1 발현에 미치는 영향
Effect of 17-beta Estradiol on Adipocyte Lipin-1 Expression in OLETF Rat
Effect of 17-beta Estradiol on Adipocyte Lipin-1 Expression in OLETF Rat
Document Type
Article
Author
강은석 / Eun Seok Kang; 김인숙 / In Sook Kim; 고석진 / Seok Jin Ko; 김철훈 / Chul Hoon Kim; 전성완 / Sung Wan Chun; 안철우 / Chul Woo Ahn; 차봉수 / Bong Soo Cha; 이현철 / Hyun Chul Lee
Source
Endocrinology and Metabolism(구 대한내분비학회지). Sep 30, 2010 25(3):199
Subject
Language
Korean
ISSN
2093-596X
Abstract
배경: 17베타-에스트라디올이 포도당 항상성유지에 중요한 역할을 한다고 사료되고 있으나 명확한 기전은 밝혀지지 않았다. Lipin은핵 내 단백질로 지방세포 분화에 있어서 필수적인 인자로 알려져 있으며 이소성 지방침착과 지방의 재분포에 관여할 것으로 사료된다. 본 연구에서는 17-베타 에스트라디올이 당뇨병 동물모델인 OLETF쥐에서 지방세포의 lipin 발현에 미치는 영향을 분석하고자 하였다. 방법: 생후 6주에 OLETF 쥐를 3군으로 나누어 한 군은 겉보기수술을 시행하고(SHAM), 다른 한 군에서는 거세하고(CAST) 나머지 한 군에서는 거세한 후 에스트로겐치료를 하였다(EST). LETO 쥐 8마리를 대조군(LETO)으로 사용하였다. EST군에서는 estradiol valerate를 0.1 mg을 한 달에 1회 6주부터 42주까지 피하주사를 실시하였다. 결과: SHAM군에서는 체중이 증가하다 고혈당증이 나타나는 30주 이후 체중증가 정도가 감소되었고 CAST군에서는 지속적으로 체중이 증가하였다. EST군에서는 체중증가 속도가 느려져서 대조군인 LETO군과 비슷한 수준으로 체중이 유지되었다. EST군에서 포도당부하검사상 혈당상승 정도가 유의하게 적었다. 내장지방세포에서의 lipin-1의 발현은 SHAM군, CAST군에서는 감소하였으나 EST군에서는 회복됨이 관찰되었다. 피하 지방세포에서의 lipin-1의 발현은 SHAM군, CAST군, EST군 모두에서 감소하였다. 결론: OLETF 쥐에서 17-베타 에스트라디올을 장기간 투여한 결과 체중증가 정도가 감소되었고 포도당부하검사에서 혈당상승 정도가 유의하게 적었으며 내장지방세포에서 lipin-1의 발현을 회복시켜 주었으나 이러한 효과는 피하지방세포에서는 관찰되지 않았다.
Background: 17 beta-estradiol is known to play an important role in glucose homeostasis. Lipin-1 is a nuclear protein that is essential in adipocyte differentiation and it is considered to play a role in ectopic fat deposition and the redistribution of fat. The aim of this study was to evaluate the effect of 17 beta-estradiol on the lipin-1 expression in the adipocytes of OLETF rats, which is an animal model of diabetes. Methods: The OLETF rats were divided into 3 groups, 1) the sham-operation group (SHAM) 2) the castrated group (CAST) and 2) the castrated and estradiol treatment group (EST), and all the rats were at 6 weeks of age. LETO rats were used as a control group (LETO). 0.1 mg of estradiol valerate was injected subcutaneously every 4 weeks in the rats of the EST group. The visceral and subcutaneous tissues were isolated to evaluate the lipin-1 protein expression. The lipin-1 expression was measured in human visceral and subcutaneous preadipocytes. Results: Less body weight gain was observed in the EST group compared with that of the SHAM group. In addition, improvement in the glucose tolerance was observed in the EST group. The lipin-1 expression in visceral fat was decreased in the SHAM and CAST groups, but it was but recovered in the EST group. The lipin-1 expression in the subcutaneous fat was decreased in the SHAM, CAST, and EST groups. Conclusion: Long term estradiol treatment in OLETF rats reduces the body weight gain and improves the glucose tolerance. Estradiol enhances the lipin-1 protein expression in the visceral adipocytes, but not in the subcutaneous adipocytes. (Endocrinol Metab 25:199-205, 2010)
Background: 17 beta-estradiol is known to play an important role in glucose homeostasis. Lipin-1 is a nuclear protein that is essential in adipocyte differentiation and it is considered to play a role in ectopic fat deposition and the redistribution of fat. The aim of this study was to evaluate the effect of 17 beta-estradiol on the lipin-1 expression in the adipocytes of OLETF rats, which is an animal model of diabetes. Methods: The OLETF rats were divided into 3 groups, 1) the sham-operation group (SHAM) 2) the castrated group (CAST) and 2) the castrated and estradiol treatment group (EST), and all the rats were at 6 weeks of age. LETO rats were used as a control group (LETO). 0.1 mg of estradiol valerate was injected subcutaneously every 4 weeks in the rats of the EST group. The visceral and subcutaneous tissues were isolated to evaluate the lipin-1 protein expression. The lipin-1 expression was measured in human visceral and subcutaneous preadipocytes. Results: Less body weight gain was observed in the EST group compared with that of the SHAM group. In addition, improvement in the glucose tolerance was observed in the EST group. The lipin-1 expression in visceral fat was decreased in the SHAM and CAST groups, but it was but recovered in the EST group. The lipin-1 expression in the subcutaneous fat was decreased in the SHAM, CAST, and EST groups. Conclusion: Long term estradiol treatment in OLETF rats reduces the body weight gain and improves the glucose tolerance. Estradiol enhances the lipin-1 protein expression in the visceral adipocytes, but not in the subcutaneous adipocytes. (Endocrinol Metab 25:199-205, 2010)