부산대학교 도서관

Background: Macrophage Migration Inhibitory Factor (MIF) is known as pro-in- fi ammatory and pro-oncogenic cytokine. Its role in gastric cancer has been revealed recently. We evaluated the relationship between MIF and H. pylori in the gastric carcinogenesis. Methods: Human gastric tissue samples from 522 patients were subdivided into 6 groups according to H. pylori infection status and pathologic diagnosis (cancer, dysplasia, or control). Tissue MIF mRNA level was measured by real-time PCR, shown as 2^delta-delta CT (2^ddCT). Results: Gastric cancer tissue expressed signifi cantly higher level of MIF mRNA (n=214, mean±SE; 9.16±1.5) than non-cancer counterpart (n=308, 4.22±0.9, p=0.005). There was no signifi cant difference in MIF mRNA level between H. pylori negative and positive tissues. In subgroup analysis, H. pylori-positive cancer tissue showed signifi cantly higher MIF expression (n=137, 10.27±2.1) than that of H. pylori-positive dysplasia (n=55, 1.91±0.5, p<0.05) or H. pylori-positive control (n=113, 3.29±0.6, p<0.05). However, no such fi nding was noted among the H. pylori-negative cancer/dysplasia/control group. (fi gure) Conclusions: Tissue MIF mRNA level showed signifi cant difference between gastric cancer and dysplasia only in H. pylori-positive group. From these results we can conclude that MIF could be an important factor in the H. pylori induced gastric carcinogenesis. Now the experiments regarding underlying mechanism are undergoing.