학술논문
정상 심근과 확장성 심근증 심근에서의 미토콘드리아 DNA 에 대한 분석
The Analysis of Mitochondrial DNA in the Failing and Normal Hearts
The Analysis of Mitochondrial DNA in the Failing and Normal Hearts
Document Type
Article
Author
최현석 / Hyun Seok Choi; 김효수 / Hyo Soo Kim; 오병희 / Byung Hee Oh; 이명묵 / Myoung Mook Lee; 최성준 / Seong Choon Choe; 홍석근 / Suk Keun Hong; 손대원 / Dae Won Sohn; 박영배 / Young Bae Park; 최윤식 / Yun Shik Choi; 서정돈 / Jung Don Seo; 이영우 / Young Woo Lee
Source
대한내과학회지 (Korean J Med) / The Korean Journal of Medicine (Korean J Med). Sep 01, 1997 53(3):404
Subject
Language
Korean
ISSN
1738-9364
Abstract
Objective: The aim of this study is to analyze the mitochondrial DNA in failing and normal hearts. Methods: Genomic DNA was extracted from 18 failing and 4 normal hearts. The DNA was digested with each 50 units of BamH I, Pvu II, Pst I, and hybridized using DNA fragments encoding CO II (cytochrome oxidase II) and CO IU. They were detected using 'Fluorescein Gene Images' system. Results: The light microscopic feature of failing myocardium was compatible with that of primary cardiomyopathy. In southern blot analysis, there was no significant difference in mitochondrial DNA amounts between normal and failing hearts. The amount of mitochondrial DNA in hearts, whether normal or failing, was greater than that in lymphocytes. There were no abnormal bands except 16.6kb-normal band using the enzyme BamH I, Pvu II from failing and normal hearts. After digesting with Pst I, 2.1kb band was found using probe CO II and 14.5kb band using probe CO III. Conclusion : The amount of mitochondrial DNA in hearts, whether normal or failing, was greater than that in lymphocytes, which suggests that the heart is an active organ in the energy metabolism. Abnormal band was not found in southern blot analysis of the mitochondrial DNA from failing and normal hearts. The more sensitive method such as PCR is required to detect the presence of sma11 amount of mutated DNA.