학술논문
Comprehensive profiling of DNA methylation in Korean patients with colorectal cancer
Document Type
Article
Author
Hyeran Shim; Kiwon Jang; Yeong Hak Bang; Hoang Bao Khanh Chu; Jisun Kang; Jin-young Lee; Sheehyun Cho; Hong Seok Lee; Jongbum Jeon; Taeyeon Hwang; Soobok Joe; Jinyeong Lim; Ji-hye Choi; Eun Hye Joo; Kyunghee Park; Ji Hwan Moon; Kyung Yeon Han; Yourae Hong; Woo Yong Lee; Hee Cheol Kim; Seong Hyeon Yun; Yong Beom Cho; Yoon Ah Park; Jung Wook Huh; Jung Kyong Shin; Dae Hee Pyo; Hyekyung Hong; Hae-ock Lee; Woong-yang Park; Jin Ok Yang; Young-joon Kim
Source
BMB Reports / Biochemistry and Molecular Biology Reports. Feb 28, 2024 57(2):110
Subject
Language
English
ISSN
1976-6696
Abstract
Alterations in DNA methylation play an important pathophysiological role in the development and progression of colorectal cancer. We comprehensively profiled DNA methylation alterations in 165 Korean patients with colorectal cancer (CRC), and conducted an in-depth investigation of cancer-specific methylation patterns. Our analysis of the tumor samples revealed a significant presence of hypomethylated probes, primarily within the gene body regions; few hypermethylated sites were observed, which were mostly enriched in promoter-like and CpG island regions. The CpG Island Methylator Phenotype- High (CIMP-H) exhibited notable enrichment of microsatellite instability-high (MSI-H). Additionally, our findings indicated a significant correlation between methylation of the MLH1 gene and MSI-H status. Furthermore, we found that the CIMP-H had a higher tendency to affect the right-side of the colon tissues and was slightly more prevalent among older patients. Through our methylome profile analysis, we successfully verified the methylation patterns and clinical characteristics of Korean patients with CRC. This valuable dataset lays a strong foundation for exploring novel molecular insights and potential therapeutic targets for the treatment of CRC.