학술논문
팥(Vigna angularis (Willd.) Ohwi & H. Ohashi) 품종의 화학적 마커와 비교하여 항당뇨병 활성에 영향을 미치는 요인을 식별하기 위한 대사체학 접근법
A metabolomics approach to identify factors influencing their α-glucosidase and hDPP-IV activity relative to chemical marker in adzuki bean (Vigna angularis (Willd.) Ohwi & H. Ohashi) cultivars
A metabolomics approach to identify factors influencing their α-glucosidase and hDPP-IV activity relative to chemical marker in adzuki bean (Vigna angularis (Willd.) Ohwi & H. Ohashi) cultivars
Document Type
Article
Author
오선민 / Seon Min Oh; 안종민 / Jongmin Ahn; 정선인 / Sunin Jung; 박지윤 / Ji-yoon Park; 김두영 / Doo-young Kim; 김정희 / Jung-hee Kim; 송석보 / Seok Bo Song; 주영광 / Young Kwang Ju; 추지호 / Ji Ho Chu; 김문옥 / Mun-ock Kim; 이병원 / Byong Won Lee; 류형원 / Hyung Won Ryu
Source
Journal of Applied Biological Chemistry. Dec 31, 2023 67:105
Subject
Language
Korean
English
English
ISSN
1976-0442
Abstract
기능성식품인 팥의 대사산물 변화와 생물학적 활성에 미치는 영향을 관찰하였다. ESI (electrospray ionization) 모드의 다변량 통계 분석을 통해 팥 품종들을 성공적으로 구별하였다. PCA 분산형 플롯, 화산 플롯 및 상자 플롯에서 볼 수 있듯이 α-glucosidase는 355.1271, 342.1189, 666.2219, 1133.5381 m/z에서 파생된 중요한 화학적 마커를 나타낸 반면, hDPP-IV는 666.2220, 342.1193 m/z에서 파생된 잠정적인 화학적 마커를 나타내었다. 또한 팥 추출물은 2차대사산물 조성에 따라 α-glucosidase와 hDPP-IV 를 다르게 억제하였다. 세부적으로 밀양 59, 해오름, 홍다, 홍연은 600 μg/mL에서 α-glucosidase를 각각 29.3, 16.6, 139.9, 20.6% 억제한 반면에 밀양59, 중원, 해오름, 홍미인은 hDPP-IV를 600μg/mL에서 각각 15.0, 21.0, 15.0, 20.0% 억제하였다. 중요한 것은 팥 품종별 차별성이 나타났으며, 이는 밀양59와 해오름의 아즈키사포닌 VI 및 그 유도체들과 강한 상관관계가 있다는 것이다.
The metabolite changes in adzuki bean, as functional food, and their effects on its biological activity were monitored. The in adzuki bean cultivars were successfully distinguished by multivariate statistical analysis in electrospray ionization mode. As shown by the PCA scatter plot, volcano plot and box plot, the α-glucosidase exhibited significant chemical markers derived from 355.1271, 342.1189, 666.2219, and 1133.5381 m/z, whereas the hDPP-IV exhibited tentative chemical markers derived from 666.2220 and 342.1193 m/z. The adzuki bean extracts also inhibited α-glucosidase and hDPP-IV differently depending on the secondary metabolites composition. In detail, Milyang59, Haeoreum, Hongda, and Hongyeon inhibited α-glucosidase by 29.3, 16.6, 139.9, and 20.6% at 600 μg/mL, respectively, while Milyang59, Joongwon, Haeoreum and Hongmiin inhibited hDPPIV by 15.0, 21.0, 15.0, and 20.0% at 600 μg/mL, respectively. Importantly, discrimination was shown across the cultivars of the adzuki bean, which correlated strongly with the azukisaponin VI and their derivatives in Milyang59 and Haeoreum.
The metabolite changes in adzuki bean, as functional food, and their effects on its biological activity were monitored. The in adzuki bean cultivars were successfully distinguished by multivariate statistical analysis in electrospray ionization mode. As shown by the PCA scatter plot, volcano plot and box plot, the α-glucosidase exhibited significant chemical markers derived from 355.1271, 342.1189, 666.2219, and 1133.5381 m/z, whereas the hDPP-IV exhibited tentative chemical markers derived from 666.2220 and 342.1193 m/z. The adzuki bean extracts also inhibited α-glucosidase and hDPP-IV differently depending on the secondary metabolites composition. In detail, Milyang59, Haeoreum, Hongda, and Hongyeon inhibited α-glucosidase by 29.3, 16.6, 139.9, and 20.6% at 600 μg/mL, respectively, while Milyang59, Joongwon, Haeoreum and Hongmiin inhibited hDPPIV by 15.0, 21.0, 15.0, and 20.0% at 600 μg/mL, respectively. Importantly, discrimination was shown across the cultivars of the adzuki bean, which correlated strongly with the azukisaponin VI and their derivatives in Milyang59 and Haeoreum.