부산대학교 도서관

Epidermal lipids within the stratum corneum (SC) are pivotal for maintaining the skin barrier function, serving to safeguard against microbial and allergen infiltration, as well as mitigating transcutaneous evaporative water loss (TEWL). In this study, we have demonstrated the novel role of α(1,2)-fucosylation by fucosyltransferase 1 (Fut1) within the granular layer of the skin in modulating epidermal lipids within the SC. Lipidomic analysis revealed diminished levels of epidermal lipid components, including ceramides, fatty acids, and triglycerides, in Fut1-deficient mice in comparison to WT mice. Furthermore, a decrease in the number of lamella bodies, crucial for epidermal lipid secretion, was observed within the granular layer of Fut1-deficient mice compared to WT counterparts. Additionally, we provided evidence of α(1,2)-fucosylation on lysosomal-associated membrane protein 1 (LAMP-1), a structure membrane protein of lamella bodies potentially involved in their biogenesis, as confirmed by immunostaining and immunoprecipitation analyses. Finally, we observed that following tape-stripping, Fut1-deficient mice exhibited heightened TEWL relative to WT mice. These findings underscore the significant role of α(1,2)-fucosylation in the regulation of epidermal lipids, which is essential for maintaining skin barrier functions.