부산대학교 도서관

Objective: Diabetes mellitus (DM) is a metabolic disorder, characterized through severe hyperglycaemia along with altered fat, protein and carbohydrate metabolism chiefly results from defects in insulin secretion/insulin action or both. Arsenic is a naturally occurring heavy metal that induced the diabetes. During the diabetes, arsenic induce the oxidative stress mediated destruction in insulin signalling in rodent. In the current study, naringenin could attenuated the streptozotocin (STZ) induced hyperglycaemic condition via impaired insulin signalling in rats. Methods: Intraperitoneal injection of STZ was used for induced the DM. Body weight, biochemical, antioxidant and hepatic parameters were determined at regular interval. STZ treatment used for the estimation of hepatocytes, β-cells, ROS accumulation and cytodegenerative effect were assayed. RT-PCR techniques were used for the estimation of underlying mechanism Results: Naringenin treatment significantly (P < 0.001) reduced the blood glucose level (57%) and oxidative stress by alteration in the activity of CAT (54%), SOD (58%), GPx (43%) and MDA (43%), respectively. Naringenin also improved the plasma insulin level (63%) brought down after STZ treatment. Naringenin also reduced the STZ induced cyto-degeneration of pancreatic β-cells (43%) and hepatocytes, and helped to scavenge the free radicals. Naringenin further reduced the level of TNF-α (54%) and IL-6 (45%), IL-1β (43%) with up-regulation of IRS-1 (23%), IRS-2 (34%), GLUT4 (54%), AKT (23%), PI3K (36%) and PPARϒ (34%) signalling molecules at mRNA and protein levels. Conclusion: Collectively, we can say that naringenin possesses and anti-diabetic effect and enhance the diminished insulin signaling in arsenic intoxicated rat.