부산대학교 도서관

Background: Acral Melanoma (AM) is a subtype of melanomas that preferentially occurs on hands and feet. It is of particular interest to understand melanoma pathogenesis, because it has different genetic factors from other melanoma subtypes and treatment. Therefore a thorough study about genomic characteristics of AM is needed to develop new treatment options. We preliminarily investigated the gene expression profiles of melanomas. Objectives: In this study, we tried to compare genomic patterns of melanomas with normal tissues and also compare gene expressions of AM with those of superficial spreading melanomas (SSM). Methods: We obtained fresh frozen tissue samples from AM and SSM, as well as normal skin and carried out cDNA microarray analysis. We also compared the gene expressions of AM, SSM, and normal tissues by RT-PCR. Results: We identified 742 up-regulated and 1518 down-regulated probe sets in melanoma compared to normal skin. Top 20 up-regulated genes in melanoma compared to normal skin include MLANA, PMEL, TYR, C6orf218, TRPM1, PRAME, RGS1. Conclusion: We collected data about differences in gene expression between melanomas and normal tissues, and also between AM and SSM. With this information we look forward to finding out specific genomic traits of AM and developing new biologic targets treating AM.