학술논문
미네랄 워터를 이용한 블랙티의 효율적 추출 방법 및 추출물의 항산화와 항염 효능
Efficient Extraction Method of Black Tea using Mineral Water and Antioxidant and Anti-inflammatory Activity of Extract
Efficient Extraction Method of Black Tea using Mineral Water and Antioxidant and Anti-inflammatory Activity of Extract
Document Type
Article
Author
김은미 / Eun-mi Kim; 정지용 / Jiyong Jung; 김형민 / Hyung-min Kim; 황경환 / Kyeong Hwan Hwang; 김형준 / Hyoung-june Kim; 박원석 / Won-seok Park
Source
대한화장품학회지(J. Soc. Cosmet. Scientists Korea) / journal of the society of cosmetic scientists of korea. Mar 30, 2022 48(1):47
Subject
Language
Korean
English
English
ISSN
1226-2587
Abstract
본 연구에서는 블랙티에 함유된 고분자 카테킨 성분을 효율적으로 추출할 수 있는 조건을 최적화하였다. 발효도에 따른 고분자 카테킨 함량을 확인하기 위하여 세가지 발효도의 블랙티내 고분자 카테킨 함량을 비교하였다. 발효도가 증가할수록 고분자 카테킨 함량이 증가되며 그중 크기가 가장 큰 데아브론닌(theabrownin) 함량이 크게 증가함을 확인하였다. 또한, 경수인 미네랄 워터를 사용하여 추출시 다른 추출수를 사용했을 때 비해 약 150% 이상 데아브로닌의 추출 햠량이 증가됨을 확인하였다. 최적 조건에서 추출된 블랙티 추출물과 그 추출물내 다량으로 함유된 데아브로닌의 항산화 효능을 ABTS assay를 이용하여 확인하였다. 그 결과, 두 추출물 모두 항산화 효능을 갖고 있으며, IC50 값이 각각 10.60 ppm, 13.21 ppm으로 확인하였다. 또한, 주요 성분인 데아브로닌의 항염 효능을 확인한 결과 UVB 조사에 의해 증가된 IL-8의 mRNA 발현이 데아브로닌에 의해 농도 의존적으로 억제됨을 확인하였다. 또한, 데아브로닌 10 ppm을 처리하면 UVB 조사에 의해 저하된 미토콘드리아의 기능이 대조군 대비 86 ± 1.9%으로 회복되었다. 따라서, 데아브로닌은 UVB 조사에 의해 유발된 미토콘드리아의 손상을 방지할 수 있음을 확인되었다.
In this study, the conditions for efficiently extracting polyphenols contained in black tea were optimized. In order to confirm the polyphenol content according to the degree of fermentation, the polyphenol content in black tea of the three fermentation degrees was compared. As the degree of fermentation increased, the polyphenol content increased, and it was confirmed that the theabrownin content, the largest in size, increased significantly. In addition, it was confirmed that the amount of theabrownin extraction increased by about 150% or more compared to other extraction water when extracting using mineral water, which was hard water. The antioxidant effects of black tea extract and the theabrownin was confirmed using ABTS assay. As a result, both extracts had antioxidant effects, and IC50 values were confirmed to be 10.60 ppm and 13.21 ppm, respectively. And also, the anti-inflammatory effect of the theabrownin was confirmed that the mRNA expression of IL-8 increased by UVB irradiation was inhibited in a concentration dependent manner by the theabrownin. In addition, when theabrownin was treated with 10 ppm, the mitochondrial function decreased by UVB irradiation was restored to 86 ± 1.9% compared to the control group. Therefore, we concluded that theabrownin could protect mitochondrial damage caused by UVB irradiation.
In this study, the conditions for efficiently extracting polyphenols contained in black tea were optimized. In order to confirm the polyphenol content according to the degree of fermentation, the polyphenol content in black tea of the three fermentation degrees was compared. As the degree of fermentation increased, the polyphenol content increased, and it was confirmed that the theabrownin content, the largest in size, increased significantly. In addition, it was confirmed that the amount of theabrownin extraction increased by about 150% or more compared to other extraction water when extracting using mineral water, which was hard water. The antioxidant effects of black tea extract and the theabrownin was confirmed using ABTS assay. As a result, both extracts had antioxidant effects, and IC50 values were confirmed to be 10.60 ppm and 13.21 ppm, respectively. And also, the anti-inflammatory effect of the theabrownin was confirmed that the mRNA expression of IL-8 increased by UVB irradiation was inhibited in a concentration dependent manner by the theabrownin. In addition, when theabrownin was treated with 10 ppm, the mitochondrial function decreased by UVB irradiation was restored to 86 ± 1.9% compared to the control group. Therefore, we concluded that theabrownin could protect mitochondrial damage caused by UVB irradiation.