학술논문

Original Article: Food Science/Microbiology : Isolation, Identification, and Characterization of a Keratin-degrading Bacterium Chryseobacterium sp. P1-3
Document Type
Article
Source
Journal of Applied Biological Chemistry. Sep 30, 2015 58(3):247
Subject
Chryseobacterium sp
isolation
keratin
Language
Korean
English
ISSN
1976-0442
Abstract
In this study, a keratin-degrading bacterium was isolated from soil contaminated with feather waste. The isolated strain was identified as Chryseobacterium sp. P1-3 on the basis of the 16S rRNA gene sequence alignment. Chryseobacterium sp. P1-3 is currently used in various biotechnological applications (e.g., in the hydrolysis of poultry feathers). It hydrolyzed the feather meal within 2 days and possesses a high level of keratinase activity (98 U/mL). The keratinase, partially purified from this strain, prefers casein as a substrate and shows optimal activity at a temperature of 30oC and at a pH of 8.0.