부산대학교 도서관

Objective: Diabetes mellitus is a metabolic disorder characterized by disturbances in carbohydrate, fat and protein metabolisms. Herbal medicines have been used in the treatment of Diabetes mellitus and various other problems for a long time in folk system of medicine. The present study was performed to understand the effect of Gymnema sylvestre in insulin-mediated glucose utilization pathways by in silico and in vivo methods. Methods: In silico analysis : G. sylvestre active constituents were docked against important molecules involved in the insulin signalling pathway like IRS-1, Akt and GSK3-β by using PatchDock and FireDock, viewed by Acceryls discovery studio visualizer. In vitro analysis: Studies were carried out in L6 skeletal muscle cell with two different concentrations of G. sylvestre. After appropriate incubation, western blotting analysis was performed to analysis the phosphorylation of the molecules. The data was subjected to one-way ANOVA (Graph Pad Prism version 6). P < 0.05 was considered statistically significant Results: G. sylvestre molecule (Gymnemagin and gymnemic acids) binds with the inhibitory sites of PTP1B, GSK3-β and it also had allosteric binding with Akt /PKB. The binding energy and the interaction with amino acid molecule were similar to known inhibitor and modulator molecules. G. sylvestre significantly stimulates the phosphorylation of IRS-1, Akt and GSK3-β upon exposure in L6 skeletal muscle cell and it was statistically significant Conclusion: G. sylvestre therapy reduced the blood glucose levels by activating and inhibiting the various enzymes through insulin signaling pathway.