학술논문
사람피부와 돼지피부를 이용한 OECD Guideline 428에 따른 피부흡수시험법 검증
Skin Absorption Test According to OECD Guideline 428 Using Human and Porcine Skin
Skin Absorption Test According to OECD Guideline 428 Using Human and Porcine Skin
Document Type
Article
Author
한주희 / Ju Hee Han; 석승혁 / Seung Hyeok Seok; 백민원 / Min Won Baek; 김동재 / Dong Jae Kim; 나이랑 / Yi Rang Na; 박귀례 / Kui Lea Park; 손경희 / Kyung Hee Sohn; 주정흔 / Jung Hun Ju; 임채형 / Chae Hyung Lim; 박재학 / Jae Hak Park
Source
한국동물실험대체법학회지 (jaae) / Journal of Alternatives to Animal Experiments. Mar 31, 2009 3(1):31
Subject
Language
Korean
ISSN
1975-9657
Abstract
In vitro skin absorption test has been proposed as a reliable alternative method to in vivo skin absorption test. In many other countries, in vitro skin absorption has been conducted according to OECD guideline which was adopted in 2000. However, in vitro skin absorption test hasn`t been validated yet in Korea. In this study, experimental conditions were set and validated according to a detailed protocol of OECD guideline 428. We compared penetration properties of caffeine (log P -0.01) and benzoic acid (log P 1.83) through human split-thickness skin and pig ear full-thickness skin using Franz cell. The permeation of caffeine through the human skin (10.16%) was higher than through pig ear full-thickness skin (1.68%). The permeation of benzoic acid through human skin and pig ear skin were similar. Hydrophilic caffeine penetrated the human split-thickness skin more efficiently than pig ear full-thickness skin. Our data showed a similar pattern with compared other reports that regarded skin absorption of caffeine and benzoic acid through split-thickness skin and full-thickness skin. In conclusion, we validated in vitro skin absorption test in Korea