학술논문
우고닌-하이드로젤 지지체의 파골세포 분화 억제 효과 / Inhibitory effects of wogonin mixed with hydrogel on osteoclast differentiation
Document Type
Dissertation/ Thesis
Author
Source
Subject
Language
Korean
Abstract
Objective: Wogonin is an active component isolated from scutellariae radix. This study was conducted to evaluate the inhibitory effect of wogonin mixed with hydrogel on osteoclast differentiation.Materials and Method: It was performed to estimate cytotoxicity of Wogonin alginate hydrogel disk (WHD) in BMMs stimulated with RANKL, M-CSF. ROS synthesis and actin ring formation were analysed to observe the effect of WHD.Results: WHD has no cytotoxicity at the concentration of 0.1㎍/㎖ or lower. 0.1㎍/㎖, 1㎍/㎖ WHD restrained the synthesis of ROS and 0.1㎍/㎖, 1㎍/㎖ WHD restrained the formation of actin ring.Conclusion: WHD has the inhibitory effect of osteocalst differentiation and bone resorption. Further studies are needed to treat osteoporosis by herbal medicine.Key Words: Scutellariae Radix, Wogonin alginate hydrogel disk, osteoclast, hydrogel, RANK ligand