부산대학교 도서관

PURPOSE: Naringenin and endurance exercise both serve as catalysts for mitochondrial biogenesis, augmenting energy provision, stabilizing metabolism, facilitating muscle adaptation, and bolstering overall health. Yet, the synergistic potential of combining naringenin with endurance exercise to further improve mitochondrial biogenesis remains largely unexplored. Hence, this study endeavors to elucidate the combined effects of naringenin and endurance exercise on augmenting mitochondrial biogenesis in the skeletal muscle of mice. METHODS: Sixteen male C57BL/6J mice were randomly divided into four groups: SED, EX, NAR, and EX+NAR after a week of acclimatization. SED groups received daily oral placebo at 8:00 AM, with no specified exercise program. EX groups had the same oral administration as the SED group, but also engaged in 8 weeks of low to moderate-intensity treadmill training. NAR groups were administered naringenin daily for 8 weeks, with no specified exercise program. EX+NAR groups followed the same administration as the NAR groups and the same exercise program as the EX groups. Mice were weighed and dissected 18 hours after the final exercise. Gastrocnemius muscle samples were frozen in liquid nitrogen and stored at -80°C. Western blot assessed factors and enzymes related to muscle mitochondrial biogenesis (PGC- 1α, NRF-1, Tfam, NADH, COX-1, and ATPsyn). SPSS 26.0 analyzed the data with means ± SEM for intragroup differences. One-way ANOVA compared intergroup differences, with Tukey’s post hoc testing (p < 0.05). RESULTS: Upon comparing factors associated with mitochondrial biogenesis and ETC enzyme levels in the EX+NAR group with the SED, EX, and NAR groups, the following findings emerged: Comparing the EX+NAR group to the SED group, NRF-1 and Tfam content increased significantly, with a slight rise in PGC-1α. COX-1 and ATPsyn content significantly exceeded that of the SED group, while NADH showed a slight increase. In comparison to the EX group, the EX+NAR group had significant differences in Tfam and NRF-1 content, with no significant change in PGC-1α. ATPsyn levels remained largely unchanged, with slight increases in NADH and COX-1. Compared to the NAR group, the EX+NAR group showed significant alterations in PGC-1α and Tfam content, a slight increase in NRF-1, and significant changes in NADH and COX-1 content, with a slight non-significant increase in ATPsyn content. CONCLUSIONS: The synergistic interaction between naringenin and exercise can enhance mitochondria biogenesis, rendering it a more effective approach to promoting health.